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Image Search Results
Journal: NPJ Regenerative Medicine
Article Title: Bioartificial pulsatile cuffs fabricated from human induced pluripotent stem cell-derived cardiomyocytes using a pre-vascularization technique
doi: 10.1038/s41536-022-00218-7
Figure Lengend Snippet: a Schematic overview of the protocol used to construct the vascular bed. GFs, growth factors. b A gelatin sheet before incubation with growth factors. c The gelatin sheet after incubation with growth factors at 4 °C for 24 h. d Azan staining of the gelatin sheet before incubation. e The vascular bed (VB) in the GF(−) group (gelatin sheet not pre-loaded with growth factors) 21 days after incubation in vivo. f The vascular bed (VB) in the GF(+) group (gelatin sheet pre-loaded with growth factors) 21 days after incubation in vivo. g Section of the vascular bed (VB) in the GF(−) group stained with hematoxylin and eosin (HE) on day 21. h Section of the vascular bed (VB) in the GF(+) group stained with HE on day 21. The region around the SFA and SFV contained new blood vessels rich in smooth muscle. i, k Fluorescent beads (4 μm diameter) were administered into the SFA and vascular bed via a catheter inserted through the abdominal aorta and advanced into the femoral artery (day 21). New blood vessels in the gelatin gel were more extensive in the FA(+) GF(+) group (gelatin sheet pre-loaded with growth factors, SFA occluded after 14 days of incubation in vivo) than in the FA(−) GF(+) group or FA(+) GF(−) group. l , m Tomato lectin (to label vascular endothelial cells) and fluorescent beads (to reveal blood flow) were administered to a vascular bed from the FA(+) GF(+) group and observed using two-photon microscopy. Endothelial cells in the gelatin sheet formed a network of vascular structures through which fluorescent beads passed. n , o Tomato lectin (to label vascular endothelial cells) and Hoechst solution (to label nuclei) were administered to a vascular bed from the FA(+) GF(+) group and observed using two-photon microscopy. The new blood vessels contained structures resembling tip cells and stalk cells. Stained nuclei were observed at the tips of the extending blood vessels. p Capillary density in the vascular bed (mean ± SEM). q Smooth muscle cell area in the vascular bed (mean ± SEM).
Article Snippet:
Techniques: Construct, Incubation, Staining, In Vivo, Microscopy